Detecting apparatus, and detecting method

ABSTRACT

A detecting apparatus for detecting information of a liquid object or sample includes a transmission path, a THz wave supplying unit, a THz wave detecting unit, and an infiltrative holding member for infiltration and holding of a liquid object. The supplying unit supplies an electromagnetic wave in a frequency range between 30 GHz and 30 THz to the transmission path. The detecting unit detects the THz wave transmitted through the transmission path. The infiltrative holding member is set at a location containing at least a portion in which an electric field distribution of the THz wave propagating along the transmission path extends.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a detecting apparatus and method fordetecting information, such as characteristics, identification,concentration, and presence or absence, of a liquid object or sample, byusing an electromagnetic wave. Particularly, the present inventionrelates to a detecting apparatus and method using an electromagneticwave at a frequency or frequencies including at least a portion of afrequency range from 30 GHz to 30 THz. The electromagnetic waveincluding at least a component in the above frequency range is called aTHz wave in this specification.

2. Description of Related Art

In recent years, techniques using a THz wave have been energeticallyresearched and developed. In particular, photon energy of a THz wave isapproximately equal to energies of molecular skeleton vibration andintermolecular action of material, and hence, techniques using a THzwave are employed in analysis of material that uses spectra, etc.obtained by the spectroscopic method.

In the above situation, “APPLIED PHYSICS LETTERS/Vol. 80, No. 1, 2002,p. 154” discloses a proposal in which a THz-wave transmission path isformed on a substrate, DNA water solution is dripped and dried on thetransmission path, and analysis of the DNA is carried out by detecting achange in a THz-wave transmission property of the transmission path. Theproposal uses the fact that a dielectric constant of single-stranded DNAfor the THz wave differs from that of double-stranded DNA, and showsthat single-stranded DNA and double-stranded DNA can be separatelyidentified based on a difference in the THz-wave transmission propertyof the transmission path.

As stated above, when optical characteristics of material, such as anabsorption coefficient, complex refractive index, and the like, areobtained by detecting a change in a transmission condition of a THz waveused, analysis, detection, identification, etc. of the material can beconducted. In the method of the above reference, however, no means isused for dripping a liquid sample onto the transmission path in animproved controllability, and accordingly it is typically not easy toaccurately drip the sample on the transmission path. The dripped liquidsample is likely to flow and spread, so a location of the dripped sampleon the transmission path and an interaction area between the THz waveand the sample are not easy to appropriately regulate.

SUMMARY OF THE INVENTION

It is therefore an object of the present invention to provide adetecting apparatus and method for detecting information of a liquidobject or sample using THz wave.

According to one aspect of the present invention, there is provided adetecting apparatus for detecting information of a liquid object orsample, which includes a transmission path, a THz wave supplying unit, aTHz wave detecting unit, and an infiltrative holding member forinfiltration and holding of a liquid sample. The supplying unit supplieselectromagnetic radiation in a frequency range between 30 GHz and 30THz, or THz wave, to the transmission path. The detecting unit detectsthe THz wave transmitted through the transmission path. The infiltrativeholding member is set at a location containing at least a portion inwhich an electric field distribution of the THz wave propagating alongthe transmission path extends.

According to another aspect of the present invention, there is provideda detecting method for detecting information of a liquid object orsample, which includes a step of preparing an apparatus including atransmission path, a THz wave supplying unit, and a THz wave detectingunit, and a second of setting an infiltrative holding member forinfiltration and holding of a liquid sample at a location containing atleast a portion in which an electric field distribution of THz wavepropagating along the transmission path extends. The supplying unitsupplies electromagnetic wave in a frequency range between 30 GHz and 30THz, or THz wave, to the transmission path. The detecting unit detectsthe THz wave transmitted through the transmission path. In the detectingmethod, the THz wave is supplied to the transmission path under acondition in which the liquid sample is infiltrated and held in theinfiltrative holding member, and the THz wave transmitted through thetransmission path is detected to detect information of the liquidsample.

According to the present invention, the infiltrative holding member forinfiltration and holding of a liquid object or sample is used, so thelocation of the dripped liquid object or sample on the transmission pathand the interaction area between the THz wave and the liquid object orsample can be accurately and precisely regulated.

The features of the present invention will be more readily understood inconnection with the following detailed description of the embodimentsand examples of the invention in connection with the drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1A is a plan view illustrating a first embodiment and example of adetecting apparatus and method according to the present invention, whichuses an infiltrative holding member for infiltration and holding of aliquid sample.

FIG. 1B is a cross-sectional view taken along line B-B′ of FIG. 1A.

FIG. 1C is a cross-sectional view taken along line A-A′ of FIG. 1A.

FIG. 2 is a view illustrating an optical system for supplying laserlight to a transmission path of the detecting apparatus of the presentinvention.

FIG. 3 is a graph showing exemplified spectra of a THz wave detected bya detecting unit of the detecting apparatus of the present invention.

FIG. 4A is a plan view illustrating a second embodiment of a detectingapparatus and method according to the present invention, in which twosides of an infiltrative holding member (a porous material) forinfiltration and holding of a liquid sample are set by non-infiltrativemembers (resin plates).

FIG. 4B is a cross-sectional view taken along line B-B′ of FIG. 4A.

FIG. 4C is a cross-sectional view taken along line A-A′ of FIG. 4A.

FIG. 5A is a plan view illustrating a third embodiment of a detectingapparatus and method according to the present invention, in which aninfiltrative holding member (a porous material) for infiltration andholding of a liquid sample is set by bonding.

FIG. 5B is a cross-sectional view taken along line B-B′ of FIG. 5A.

FIG. 5C is a cross-sectional view taken along line A-A′ of FIG. 5A.

FIG. 6A is a plan view illustrating a fourth embodiment of a detectingapparatus and method according to the present invention, in which a flowpath is provided near an infiltrative holding member (a porous material)for infiltration and holding of a liquid sample.

FIG. 6B is a cross-sectional view taken along line B-B′ of FIG. 6A.

FIG. 6C is a cross-sectional view taken along line A-A′ of FIG. 6A.

FIG. 7A is a plan view illustrating a fifth embodiment of a detectingapparatus and method according to the present invention, in which a holeis formed in a second resin layer, and an infiltrative holding member (aporous material) for infiltration and holding of a liquid sample isdisposed in the hole.

FIG. 7B is a cross-sectional view taken along line B-B′ of FIG. 7A.

FIG. 7C is a cross-sectional view taken along line A-A′ of FIG. 7A.

FIG. 8A is a plan view illustrating a sixth embodiment of a detectingapparatus and method according to the present invention, in which adielectric layer includes an infiltrative holding member (a porousmaterial) for infiltration and holding of a liquid sample.

FIG. 8B is a cross-sectional view taken along line B-B′ of FIG. 8A.

FIG. 8C is a cross-sectional view taken along line A-A′ of FIG. 8A.

FIG. 9A is a plan view illustrating a location of an infiltrativeholding member (a porous material) relative to a transmission path.

FIG. 9B is a graph showing an electric field distribution of THz wavepropagating along the transmission path in FIG. 9A.

FIG. 9C is another plan view illustrating a location of an infiltrativeholding member (a porous material) relative to a transmission path.

FIG. 9D is a graph showing an electric field distribution of THz wavepropagating along the transmission path in FIG. 9C.

FIG. 10A is another plan view illustrating a location of an infiltrativeholding member (a porous material) relative to a transmission path.

FIG. 10B is a graph showing an electric field distribution of THz wavepropagating along the transmission path in FIG. 10A.

FIG. 10C is another plan view illustrating a location of an infiltrativeholding member (a porous material) relative to a transmission path.

FIG. 10D is a graph showing an electric field distribution of THz wavepropagating along the transmission path in FIG. 10C.

DESCRIPTION OF THE EMBODIMENTS

Embodiments and examples of a detecting apparatus and method fordetecting information of a liquid object of the present invention willhereinafter be described with reference to the drawings.

A typical embodiment of apparatus for detecting information of a liquidobject or sample includes a transmission path for transmitting THz wavetherethrough, a THz-wave supplying unit for supplying THz wave to thetransmission path through a coupling portion, and a THz-wave detectingunit for detecting the THz wave received from the transmission path viaa coupling portion. Further, there is provided an infiltrative holdingmember for infiltration and holding of a liquid sample by capillaryforce, surface tension, etc. around the transmission path, or in aportion of a dielectric layer forming the transmission path.

The infiltrative holding member is set at a location containing at leasta portion in which an electric field distribution of the THz wavepropagating along the transmission path extends. In the aboveconstruction, the detecting unit can obtain information of a change in atransmission condition of the THz wave transmitted through thetransmission path caused by constituents of the liquid sample, itspresence or absence, etc. Thus, information of the liquid sample can beacquired.

The infiltrative holding member allows the liquid sample supplied bydripping or the like to be uniformly infiltrated therein by capillaryforce, etc. and held therein. The infiltrative holding member preventsthe liquid sample from flowing out. Further, even in the event that theliquid sample dries and its solute precipitates in the infiltrativeholding member, the solute is uniformly held in the infiltrative holdingmember. Here, the wording of “uniformly” means that the sample gives asubstantially uniform action to the THz wave used. In other words, whenobserving the sample at a positional resolution of about a wavelength ofthe THz wave used, the sample uniformly behaves for the THz wave.

Accordingly, when the infiltrative holding member is accurately set, theinteraction area between the THz wave propagating along the transmissionpath and the sample held in the infiltrative holding member is virtuallyalways established in an appropriate manner. This is also assured by thefact that the liquid sample dripped on any position of the infiltrativeholding member infiltrates into the entirety thereof virtually withoutfail. Accordingly, even quantitative detection of measuring theconcentration of the sample, and the like can be accurately achieved.

A constituent material of the infiltrative holding member is preferablya material having a high transmissivity for the THz wave used. Thestructure of the infiltrative holding member can be porous, fibrous,needle-like, or the like. The dimension (i.e., diameter of porosity,thickness of fiber or distance between fibers, or thickness of needle ordistance between needles) of a fine structure thereof is desirablysmaller enough than the wavelength of the THz wave used such that itdoes not scatter the radiation.

The length of a longitudinal side of the infiltrative holding member ispreferably less than the length of the transmission path. The reasontherefor is that the interaction area between the liquid sample and theTHz wave propagating along the transmission path is regulated by thesize of the infiltrative holding member, since the liquid sampleinfiltrates almost into the entirety of the infiltrative holding memberwhen the sample is dripped on the infiltrative holding member. Further,the electric field distribution of the propagating THz wave isexceedingly weak outside the transmission path, and hence this does notcontribute to the detection. However, even an infiltrative holdingmember with a longitudinal side longer than the transmission path can beused when a mechanism for preventing infiltration of the liquid samplebeyond an area of the transmission path is formed. This is, for example,a mechanism for preventing infiltration beyond a desired region bypressing and crushing a portion of the infiltrative holding member witha frame member made of a non-infiltrative material.

As a method for infiltrating and holding a liquid sample in theinfiltrative holding member, injecting means, such as a micro-injectorand a pipette capable of dripping a minute drop of liquid, can be used.

When the amount of a liquid sample supplied onto the infiltrativeholding member exceeds its infiltrative holding ability, there is apossibility that the liquid sample comes out from the infiltrativeholding member. In such a case, it is preferable to dispose anon-infiltrative member incapable of infiltration of the liquid sample(e.g., a solid resin) adjacent to a part or the entirety of theinfiltrative holding member. Thereby, the liquid sample can be assuredlyprevented from coming out from the infiltrative holding member, and theinteraction area between the liquid sample and the THz wave can beaccurately regulated.

When the amount of dripped liquid sample exceeds the volume of a spaceformed by the non-infiltrative member, the volume of the liquid samplecan be maintained at a predetermined value by wiping and removingoverflowing liquid sample.

The non-infiltrative member can be disposed adjacent to at least aportion (e.g., two sides, or four sides) of the infiltrative holdingmember to hold it at a predetermined location near the transmissionpath. In the case of two sides, for example, the non-infiltrative memberis disposed to adjoin two sides or surfaces of the infiltrative holdingmember extending along the transmission path. In the case of four sides,the non-infiltrative member is disposed to adjoin two sides or surfacesof the infiltrative holding member extending along the transmissionpath, and two sides or surfaces thereof extending in a directiontraversing the transmission path.

When the non-infiltrative member is disposed adjacent to a portion orthe entirety of the infiltrative holding member, a spacing below awavelength of the THz wave used can be formed between the infiltrativeholding member and the non-infiltrative member. The liquid sample can besupplied to the infiltrative holding member using the spacing as aguiding flow path. In another configuration, the infiltrative holdingmember can be brought into close contact with the non-infiltrativemember.

When the non-infiltrative member is used, there is a case where anoptical constant (refractive index, absorption coefficient, or the like)of the non-infiltrative member for the THz wave used is approximatelyequal to that of the infiltrative holding member, and there is a casewhere this is not so. The former case is a case where the detection isperformed under a condition wherein THz wave propagating along thetransmission path is not strongly reflected at a boundary between theinfiltrative holding member and the non-infiltrative member. The lattercase is a case where the reflection at the boundary between theinfiltrative holding member and the non-infiltrative member ispositively used, and a THz-wave resonator or THz-wave filter isconstructed. In this latter case, the detection of the liquid sample isperformed based on a change in properties of the THz-wave resonator orTHz-wave filter caused by the supply of the liquid sample to theinfiltrative holding member.

When a metal microstrip line is used as the transmission path, theinfiltrative holding member is disposed crossing its signal line, forexample. In another case, the infiltrative holding member can be used asthe dielectric between the signal line and the ground plane of thetransmission path. It is preferable that the infiltrative holding memberis disposed exclusively in the transmission path, or a nearby regionwhere the electric field of the THz wave is relatively stronglydistributed. Thereby, an effective interaction between the liquid sampleand the THz wave can be achieved, and precision of the detection can beimproved.

For example, when the infiltrative holding member 92, such as a porousmaterial, is disposed on a plane parallel with a substrate including thesignal line 91 (the transmission path), as illustrated in FIG. 9A, theelectric field present on this plane is distributed symmetrically abouta geometrically symmetric center line of the signal line, as shown inFIG. 9B. In such a case, the interaction between the sample in theinfiltrative holding member and the THz wave propagating along thetransmission path becomes strongest when the geometrically symmetriccenter line of the electric field is approximately aligned with thegeometrically symmetric center line of the signal line. Where E(0)=1 isset in FIG. 9B, the interaction between the wave and the object canvirtually occur down to a location of E(x)=1/e². Therefore, theinfiltrative holding member should be set in a range of E(x)>1/e².Needless to say, it is best to set the object at a location of E(0)=1.FIG. 9D, FIG. 110B and FIG. 10D also show electrical fielddistributions, respectively. The infiltrative holding members havevarious shapes, and the curve of the electrical field distributionvaries depending on the shape of the infiltrative holding member.

Further, also when the infiltrative holding member is used as thedielectric between the signal line and the ground plane, the electricfield present on a plane parallel with the substrate and near the groundplane is distributed symmetrically about a geometrically symmetriccenter line parallel with the signal line. Accordingly, also in thiscase, the interaction between the sample in the infiltrative holdingmember and the THz wave propagating along the transmission path becomesstrongest when the symmetrical center line of the electric field isapproximately aligned with the symmetrical center line of the signalline.

There is a case where two symmetrical center lines of the electric fielddistribution extending in directions parallel and perpendicular to thepropagation direction of the THz wave exist. In such a case, it ispreferable that the symmetrical center line of the electric fielddistribution parallel with the propagation direction is approximatelyaligned with the symmetrical center line of the infiltrative holdingmember.

In general, observing in a plane parallel with the substrateconstituting the transmission path, the electric field distribution ofthe THz wave along the transmission path is symmetrical about a centerline parallel with the symmetrical center line of the transmission path.In such a case, the interaction between the sample in the infiltrativeholding member and the THz wave propagating along the transmission pathbecomes strongest when the symmetrical center line of the electric fielddistribution in a plane wherein the infiltrative holding member is setis approximately aligned with the center line of the infiltrativeholding member.

Further, for example, when the infiltrative holding member 93, such as aporous material, is asymmetrical as illustrated in FIG. 9C, it ispreferable that a geometrical gravity center line (a line passing acenter of gravity) of the infiltrative holding member is approximatelyaligned with the symmetrical center line of the electric fielddistribution shown in FIG. 9D.

Furthermore, for example, when the electric field distribution of theTHz wave is asymmetrical as illustrated in FIGS. 10A to 10D, it ispreferable that a geometrical gravity center line of the electric fielddistribution is approximately aligned with the symmetrical center line(in the case of FIGS. 10A and 10B), or the geometrical gravity centerline (in the case of FIGS. 10C and 10D) of the infiltrative holdingmembers 102 and 103, respectively. In FIGS. 10A and 10C, element 101depicts the signal line.

Changes in transmission condition of the THz wave due to the sampleinfiltrated in the infiltrative holding member include a change instrength of the THz wave, a change in electric field amplitude orwaveform of the THz wave, and the like. When a time domain waveform ofthe THz wave is obtained, a spectrum of the THz wave transmitted throughthe transmission path can be acquired by Fourier-transforming the timedomain waveform. The detection of the sample can be achieved based onthe change in the spectrum. Refractive index, transmissivity, absorptioncoefficient, or the like can be obtained from those changes. Thus,information of the sample, such as its identification, can be obtained.

The infiltrative holding member preferably has a high and uniformtransmissivity over the overall frequency of the THz wave propagatingthe transmission path. Since the frequency range of the THz wavepropagating the transmission path depends on properties of a THz-wavegenerating source and the structure of the transmission path, actuallyrequired characteristics of a high and uniform transmissivity varydepending on cases.

A manner of arrangement of the infiltrative holding member on thetransmission path varies depending on whether the structure (a structuredetermining the effective dielectric constant, and the like) of theinfiltrative holding member is isotropic or anisotropic for the THz wavepropagating the transmission path. Further, the manner of arrangement ofthe infiltrative holding member on the transmission path variesdepending on whether the liquid sample infiltrates in the infiltrativeholding member in an isotropic or anisotropic fashion. Morespecifically, when the infiltrative holding member is isotropic, theinfiltrative holding member can be set on the transmission path withoutconsidering its arrangement direction, and accordingly it can bearranged easily. When the infiltrative holding member is anisotropic,the infiltrative holding member needs to be set on the transmissionpath, for example, with the characteristic direction of the infiltrativeholding member being aligned with the extending direction of thetransmission path, or a direction perpendicular thereto. Thereby,reproducibility of the detection of the liquid sample can be improved.

As a material that has a high and uniform transmissivity as statedabove, there are porous materials with a granular or sponge structureformed of polypropylene, polysulfone, nylon, and polyethersulfone. Theseexamples are also isotropic infiltrative holding members. The porousmaterial with a granular structure is a material in which a large numberof fine particles disperse and are in contact with each other at pointor surface. The porous material with a sponge structure is a material inwhich a large number of fine porosities disperse in a backgroundmaterial. A porous material with a granular structure, in which a largenumber of fine particles disperse in an isotropic fashion (i.e., thecharging rate of fine particles is substantially constant in every unitregion), and which includes substantially no fibrous structure, is apreferable isotropic infiltrative holding member. Further, a porousmaterial with a sponge structure, in which a large number of fineporosities disperse in an isotropic fashion (i.e., the volumetric rateof fine porosities is substantially constant in every unit region), andwhich includes substantially no fibrous structure, is a preferableisotropic infiltrative holding member.

Inventors of the present invention performed the following experiment toobtain transmissivities of various porous materials. In this experiment,the detection was conducted by a so-called terahertz time-domainspectroscopy in which THz-wave pulses are generated from aphotoconductive antenna, the THz-wave pulses are transmitted in a space,and the THz-wave pulses are detected by another photoconductive antenna.A sheet porous material is arranged in a transmission path of theTHz-wave pulses, to obtain the transmissivity of the porous material.

According to results of the above experiment, it can be understood thatevery porous material exhibits a preferable transmissivity.Particularly, the porous materials with a granular or sponge structureformed of polypropylene, polysulfone, nylon, and polyethersulfoneexhibits the following result. The transmissivity for THz wave at 2.0THz is above 90 percent, and a value ν′ defined by the following formula(1) showing a uniformity of the transmissivity is above 20 (see thefollowing Tables 1-1 and 1-2). Here, the transmissivity is an amplitudetransmittance defined by a ratio of an absolute value of amplitude ofTHz wave after transmission through the porous material relative to thatbefore transmission through the porous material.

ν′=|T _(M)−1|/|T _(L) −T _(H)|  (1)

ν′ is equal to a value obtained by dividing a value (which is obtainedby subtracting 1 (one) from a transmissivity T_(M) at a frequency near acenter between uppermost frequency and lowermost frequency of THz waveused for measurement) by a difference between a transmissivity T_(H) atthe uppermost frequency and a transmissivity T_(L) at the lowermostfrequency, and taking an absolute value of this quotient. This valueexhibits a degree of uniformity of the transmissivity over a frequencyrange. As the value ν′ increases, the frequency dependency of thetransmissivity decreases. For example, at an extreme where the value ν′is infinite, the value ν′ is independent of the frequency. Theindication of uniformity of the transmissivity by the formula (1) isdefined with reference to Abbe's number used in the field of opticalmaterial for showing the frequency dependency of a refractive index.Hereinafter, the value ν′ will be referred to as a transmissivity Abbe'snumber.

TABLE 1-1 amplitude transmissivity porosity main material of 0.5 1.0 2.0diameter thickness membrane filter THz THz THz (micron) (micron) usepolypropylene 100 98 96 0.45 114 solution and solvent usable Polysulfone97 97 100 0.45 145 sterilization and filtration of living object andmedicine polyethersulfone 98 96 100 0.45 140 sterilization andfiltration of living object and medicine Nylon 97 96 95 0.45 127suitable for organic solvent polyvinyliden- 98 96 84 0.45 147 suitablefor difluoride protein and nucleic acid nitro cellulose 97 91 85 0.2 145western transfer Cellulose 95 89 70 20 to 25 210 for analysis of Al,etc. glass fiber 98 90 80 1.6 260 monitoring of atmospheric pollutioncellulose-mixed-ester 98 95 88 0.8 152 Microbioassay quartz fiber 98 9982 450 atomic absorption analysis and emission spectroscopy

TABLE 1-2 uniformity main material of filtration (eccentricity) Abbe'sporosity membrane filter property rate *1 number property polypropyleneadsorption of high 1.02 24.3 sponge-like low protein polysulfoneadsorption of high 1.09 32.0 sponge-like low protein polyethersulfoneadsorption of high 1.00 47.5 granular low biomolecule nylonhydrophilicity high 1.01 47.5 sponge-like of membrane itselfpolyvinyliden- resistivity to no filtration incapable of 6.79 fibrousand difluoride biosolvent estimation granular nitro cellulose highcoupling very low 1.16 7.50 fibrous ability to protein and nucleic acidcellulose for gelatin- very high 1.11 3.52 fibrous like precipitantglass fiber high filtration very high not clear 4.94 fibrous rate andholding of fine particles cellulose-mixed-ester high capture low 1.369.40 fibrous and rate sponge-like quartz fiber low background very high1.12 6.13 fibrous Note: In the column of *1, 1.0 represents a truecircle, polyvinyliden-difluoride is incapable of estimation since liquidcannot infiltrate in polyvinyliden-difluoride, and glass fiber is notclear since the boundary of liquid infiltration area is not clear.

The above granular or sponge structure can be distinguished from anon-granular or non-sponge structure by the following criteria. Where aliquid droplet is dripped on a sheet porous material, the sheet porousmaterial can be deemed to have a granular or sponge structure when aspread of the dripped droplet is approximately circular. The sheetporous material cannot be deemed to have a granular or sponge structurewhen a spread of the dripped droplet is non-circular, such aselliptical. In the case of a non-circular spread, the spread can bedetermined to be non-uniform when a value obtained by dividing a longerdiameter by a shorter diameter of the ellipse or the like is over 1.11.Therefore, with respect to boundary structures between the granular orsponge structure and the non-granular or non-sponge structure, theporous material can be deemed to have a granular or sponge structure ifthe spread shows the above value of less than 1.11.

To paraphrase the above, the porous materials with a granular or spongestructure formed of polypropylene, polysulfone, nylon, andpolyethersulfone have a relatively wide range over which thetransmissivity is high for THz wave, and can be used as a preferableinfiltrative holding material.

A porous material with a granular or sponge structure formed of materialhaving a high transmissivity for THz wave is also preferable even if thematerial is other than the above-stated polypropylene, polysulfone,nylon, and polyethersulfone. Such a porous material is considered tohave a relatively high transmissivity, and a large uniformity of thetransmissivity for THz wave.

The principal object of the infiltrative holding member is to hold theliquid sample or object. Therefore, the infiltrative holding member isnot necessarily a porous material with a granular or sponge structureformed of the above-stated polypropylene, polysulfone, nylon, orpolyethersulfone. Further, the actual frequency range of generated ordetected THz wave can be without a range between 0.5 THz and 2.0 THzdescribed in the Tables 1-1 and 1-2 because of the structure of thetransmission path and properties of the THz-wave generator. Accordingly,it is permissible to use a material having a sufficiently high anduniform transmissivity for THz wave in an actual frequency range.

The above-discussed porous material with a granular or sponge structurehas an isotropic structure, so that it can be advantageously set on thetransmission path without considering its arrangement direction. Incontrast, the porous material with a fibrous structure can improvereproducibility of the detection when it is set on the transmissionpath, for example, with its fibrous direction being aligned with theextending direction of the transmission path, or a directionperpendicular thereto. Further, when the liquid sample is dripped on theporous material with a fibrous structure, the sample is likely toinfiltrate in an elliptical form. Therefore, when a longer-diameterdirection of the ellipse is aligned with the extending direction of thetransmission path, it is possible to achieve an effective interactionbetween THz wave and the liquid sample, and improve sensitivity of thedetection.

The embodiments will be described in more detail with reference to FIGS.1A to 1C. In the detecting apparatus of the first embodiment, a groundof the transmission path is a metal plane 12 formed on a substrate 11 byvacuum evaporation or the like. The substrate 11 is, for example, asilicon substrate. The metal plane 12 is made of, for example, 500 Åthin titanium and 3000 Å thin gold. On the metal plane 12, two LT(low-temperature)-GaAs layers 13 a and 13 b are formed by epitaxiallift-off or the like. A dielectric layer 14 relatively transparent forTHz wave is formed on the LIT-GaAs layers 13 a and 13 b. The dielectriclayer 14 is made of, for example, BCB (benzocyclobutene). The thicknessof the dielectric layer 14 is 5 microns, for example. A portion of thedielectric layer 14 is removed, and the LT-GaAs layers 13 a and 13 b arepartly exposed. On the dielectric layer 14, a metal line (a signal line)15 is formed extending with a width of about 5 microns to 10 microns,and a longitudinal length of about 1 mm. Gaps 16 a and 16 b of about 5microns to 10 microns are formed in two areas along the metal line 15.Electrodes 18 a, 18 b, 18 c, and 18 d are provided at end portions ofthe metal line 15. A transmission path 101 is constructed to extend inthe extending direction of the metal line 15.

A porous material 17 is disposed on the metal line 15. The porousmaterial 17 is an infiltrative holding member for holding a liquidsample by capillary force and the like. For example, a rectangularmembrane filter is used as the porous material 17. The porous material17 is set by bonding adhesive whose loss for THz wave is small, or bypressing it against the transmission path 101 with another member. Amethod of pushing the porous material 17 against the transmission path101 with a resin plate (non-infiltrative member) 19 will be described.

In this method, a hole is formed in the polystyrene plate 19 having athickness of about 1 mm, and the porous material 17 with an outer sizeslightly larger than the hole is set covering the hole therewith, usingbonding adhesive or the like. The polystyrene plate 19 is disposed suchthat the porous material 17 crosses the metal line 15 at a locationbetween the two LT-GaAs layers 13 a and 13 b. Preferably, thesymmetrical center line of the porous material 17 is approximatelyaligned with the line 15. The reason therefor is as follows. With thetransmission path 101 of the microstrip line type as described above,the electric field of THz wave becomes stronger at a location as thelocation approaches the center of the metal line 15. Accordingly,interaction between the liquid sample held in the porous material 17 andTHz wave propagating along the transmission path 101 becomes strongerwhen the symmetrical center line of the porous material 17 isapproximately aligned with the line 15. Further, it is preferable tobring the porous material 17 into contact with the line 15 since theabove interaction increases.

A marker (alignment mark) 100 can be provided at a portion of the porousmaterial 17 or polystyrene plate 19, and a portion of the dielectriclayer 14 for the purpose of achieving such an accurate arrangement thatthe symmetrical center line of the porous material 17 is approximatelyaligned with the line 15.

The refractive index of the polystyrene plate 19 for THz wave ispreferably close to that of the porous material 17. This is because ifthose refractive indices are largely different, reflection and the likedue to impedance mismatch may occur at the boundary. For example, amembrane filter (a product by Nihon Pall Ltd.; product No. 60172) formedof hydrophilic polyethersulfone with an average porosity diameter of0.45 micron can be used as the porous material 17, and a polystyreneplate coated with hydrophobic foamy styrene can be used as thepolystyrene plate 19. In this case, the refractive index of the porousmaterial 17 is about 1.2, and the refractive index of the polystyreneplate 19 is from about 1.05 to about 1.1.

Preferably, the width of the rectangular porous material 17 isapproximately over three times as large as the width of the line 15. Thetransmission path 101 for transmitting THz wave therethrough is composedof the metal plane 12, the dielectric layer 14 and the line 15. Withthis transmission path of the microstrip line type, the electric fieldof propagating THz wave is strongly present within a region having awidth that is about three times as large as the width of the line 15.Therefore, when the width of the porous material 17 is approximately setover three times as large as the width of the line 15, the interactionbetween the sample held in the porous material 17 and the propagatingTHz wave increases. The width of the porous material 17, however, can bemade much larger in the light of fabrication convenience. As for thelongitudinal length of its side, it is preferably below the length ofthe transmission path. In this embodiment, length, width, and thicknessof the porous material 17 are, for example, about 0.6 mm, about 0.4 mm,and about 150 microns, respectively.

The average porosity diameter of the porous material 17 is, for example,0.5 micron. Further, the porous material 17 is formed of a material witha high transmissivity for THz wave (e.g., hydrophilic polyethersulfone).

Two electrodes 18 a and 18 c; 18 b and 18 d are provided at end portionsof the line 15, respectively. A voltage of about 10 V is applied throughthe electrode 18 a in the THz-wave supplying unit, and the electrode 18b in the THz-wave detecting unit is connected to a signal amplifier 110.The other electrodes 18 c and 18 d are connected to the ground. In thisembodiment, the end portions of the line 15 act as a coupler forcoupling the THz wave from the supplying unit to the transmission path,and a coupler for coupling the THz wave from the transmission path tothe detecting unit, respectively.

In this embodiment, the gap 16 a of the THz-wave supplying unit isilluminated with femtosecond (fsec) laser light to generate THz wave,and the THz wave is transmitted toward the gap 16 b of the THz-wavedetecting unit through the line 15. On the way of transmission throughthe transmission path, the THz wave interacts with the sampleinfiltrated in the porous member 17. On the other hand, the gap 16 b ofthe THz-wave detecting unit is illuminated with the fsec laser, and theTHz wave transmitted through the line 15 is detected through the signalamplifier 110.

The liquid sample, such as DNA solution, is dripped and infiltrated inthe porous member 17 using a micro-injector or the like. The liquidsample is infiltrated and held in the porous member 17 by capillaryforce and the like. The transmission condition of the THz wave detectedby the detecting unit is changed (e.g., attenuated) by the sample heldin the porous member 17, Information of the sample can be thus acquiredfrom the spectrum or the like based on the above change.

FIG. 2 illustrates a peripheral optical system which is preferably usedby the above detecting apparatus. As illustrated in FIG. 2, fsec pulselaser light emitted from a mode-locked Ti:sapphire laser (fsec laser) 21is split by a beam splitter 26. One laser light is condensed andilluminated on the gap 16 a of the THz-wave supplying unit illustratedin FIG. 1, to which the voltage is applied. The other laser light istransmitted through a time delay optical system 22 by using mirrors 27,and then condensed and illuminated on the gap 16 b of the THz-wavedetecting unit of FIG. 1, which is connected to the signal amplifier110.

The interval between gaps 16 a and 16 b in the detecting apparatus 24 isexceedingly narrow (e.g., about 1 nm). Accordingly, it is preferable tore-collect the split laser light pulses prior to the above condensationand illumination, and project the re-collected light pulses on the gapsthrough a single object lens 23 or the like, respectively. Thetime-domain waveform of the THz wave transmitted through thetransmission path 101 can be obtained when the time delay system 22 ismoved back and forth. Fourier transformation of the obtained time-domainwaveform brings forth the spectrum of THz wave whose transmissioncondition is changed according to characteristics, presence or absence,or the like of the sample. Information of the sample can be thusacquired.

In the above discussion, the porous material is used as the infiltrativeholding member, but the fibrous material and the needle-like materialcan also be used. Further, the porous material 17 is set on thetransmission path using the polystyrene plate (non-infiltrative member)19 in the above description. The infiltrative holding member, however,can be set directly on the transmission path by bonding adhesive, orthermal pressure bonding. Furthermore, the dielectric of thetransmission path can be made of a material, such as a porous material,capable of infiltration and holding of the liquid sample by capillaryforce and the like.

With respect to the structure and configuration of the transmissionpath, it is not limited to the above microstrip line. The transmissionpath can also be formed by strip line, coplanar strip line, coplanarwaveguide, micro-coplanar strip line, slab line, slot line, or the like.

In the above detecting apparatus and method, the infiltrative holdingmember for infiltration and holding of the liquid sample is used.Therefore, the location of the liquid sample relative to thetransmission path can be regulated accurately. Hence, even thequantitative detection, inspection and measurement of the sample, suchas measurement of its concentration, can be precisely achieved in animproved reproducibility. Further, when the liquid sample is dripped anddried in the infiltrative holding member, the thickness of precipitatedsample becomes uniform. The spread of the liquid sample in theinfiltrative holding member remains unchanged each time the sample isdripped, and hence the volume of interaction between the sample and THzwave does not fluctuate.

In conventional apparatuses and methods, there is a possibility that thesample drop falls, and the interaction volume between the sample and THzwave changes when the transmission path is violently handled prior tosufficient drying of the sample, for example. In the above embodiment,such possibility can be eliminated.

Thus, in the above embodiment, the desired amount of the liquid samplecan be accurately set on the transmission path, or near a desiredportion thereof, in each detection. Even the quantitative measurementcan be readily carried out in a good reproducibility. For example, aliving object, such as DNA, can be held in the infiltrative holdingmember with its three-dimensional configuration being unchanged evenwhen the liquid sample is dried. Precise information of the sample canbe acquired.

Description will be made for more specific embodiments and examples. Afirst embodiment will be described with reference to FIGS. 1A to 1C. Thefirst embodiment corresponds to the above-discussed embodiment. In thisembodiment, the porous material 17 attached to the polystyrene plate 19is prepared, and set on the line 15. The above-noted membrane filterproduced by Nihon Pall Ltd. (product No. 60172) is preferably used asthe porous material 17. When the polystyrene plate 19 with the porousmaterial 17 attached thereto is disposed on the line 15, the marker(alignment mark) 100 is preferably used. Accordingly, the porousmaterial 17 can be set on a desired location relative to the line 15.

When the liquid sample is infiltrated into the porous material 17 set onthe line 15, DNA solution is dripped on the porous material 17 through ahole in the polystyrene plate 19 using a microinjector (e.g., aprogrammable microinjector IM-300 produced by NARISHIGE SCIENTIFICINSTRUMENT LAB.). The concentration of the DNA solution is, for example,0.5 microgram/microliter. The dripped amount is, for example, 30 nl. Thedripped DNA solution is infiltrated and held in the porous material 17,and reaches a contact interface between the signal line 15 and theporous material 17. In the case the dripped amount is extremely little(e.g., 1 pl), there is a possibility that the dripped DNA solution doesnot infiltrate into the overall porous material 17. This substantiallyimpedes effective accomplishment that the interaction region between thesample and THz wave is accurately regulated. Therefore, there is a needto drip an amount enough to achieve infiltration of the liquid sample inthe entirety of the porous material 17. Further, also in the case theDNA solution is not sufficiently infiltrated in the porous material 17due to too dense concentration, too high viscosity, or the like, theeffective accomplishment cannot be obtained. In such a case, therefore,the solution should be appropriately thinned, for example. Theconcentration of the DNA solution in the porous material 17 can beincreased by repetition of dripping and drying of the sample.

The DNA solution dripped on the porous material is dried in roomtemperature. After that, the THz wave generated by the THz-wavesupplying unit is transmitted through the transmission path 101 todetect, for example, the time-domain waveform of the THz wave, accordingto the method as described in the above embodiment.

FIG. 3 shows exemplified spectra by Fourier-transforming time-domainwaveforms of THz waves transmitted through the transmission path 101 anddetected by the THz-wave detecting unit. In FIG. 3, the solid line aillustrates the exemplified spectrum of the THz wave detected before theDNA solution is dripped on the porous material 17, and the dotted line billustrates the exemplified spectrum of the THz wave detected after theDNA solution is dripped and dried. Information of presence, quantity,and so forth of the DNA can be detected based on the difference of theTHz-wave spectra detected before and after the drip of the sample, asillustrated in FIG. 3. In this embodiment, the THz-wave spectrumdetected after the drip of the sample can be obtained in a goodreproducibility, so that accurate information of the sample can beacquired. In contrast thereto, it is not easy for the conventionaltechnique to stably drip the liquid sample on an appropriate location onthe transmission path in a preferable reproducibility. Therefore,according to the conventional technique, the THz-wave spectrum detectedafter the drip of the sample cannot typically be obtained in asatisfactory reproducibility.

In the first embodiment, the DNA solution is approximately uniformlyheld in the porous material 17 set on the transmission path 101.Accordingly, reproducibility is obtained in the quantitativemeasurement, and an accurate quantitative detection is guaranteed. Thevolume of the porous material 17 is about 0.6 mm (length in a directionparallel with the signal line 15)*0.4 mm (width in a directionperpendicular to the signal line 15)*0.15 mm (thickness). The liquidsample, therefore, can be concentrated in a region over whichpropagating THz wave is distributed relatively strongly. Thus, efficientand accurate detection can be achieved.

Further, in this embodiment, a frame of the polystyrene plate(non-infiltrative member) 19 exists around the porous material 17.Hence, there is virtually no possibility that the liquid sample comesout of the porous material even if too much liquid sample is drippedbeyond liquid holding capability of the porous material 17. When theliquid sample over the volume defined by the polystyrene frame isdripped, excessive liquid sample can be wiped and removed. Thus, thedistribution of the liquid sample can be effectively regulated.

Furthermore, the detection can be repetitively performed by removing theused porous material 17 with the resin plate 19 and disposing an unusedresin plate with a porous material on the same transmission path 101.When the marker (alignment mark) 100 is provided, the relativepositional relation between the line 15 and the porous material 17 canbe stably established each time of the detection. The reproducibilitycan be thus assured.

A second embodiment of the present invention will be described referringto FIGS. 4A to 4C. Also in this embodiment, similar to the firstembodiment, the porous material 42 is set crossing the line 41 in thedetecting apparatus including the THz-wave supplying unit, thetransmission path (microstrip line structure), and the detecting unit.In the second embodiment, two non-infiltrative members or polystyreneplates 43 a and 43 b are pressed against two sides (parallel with theline 41) of the porous material 42, respectively. The porous material 42can be thus set. Other structures of the second embodiment aresubstantially the same as those of the first embodiment. In FIGS. 4A to4C, reference numerals are omitted with respect to those elements.

The polystyrene plate 43 is preferably slightly larger than the porousmaterial 41 in the longitudinal direction (e.g., 1 mm*0.6 mm). Twopolystyrene plates 43 a and 43 b are preferably spaced from each otherby more than thrice the width of the line 41 (i.e., each plate is awayfrom the center of the line 41 by more than 1.5 times the widththereof). The reason is that such arrangement can prevent an impedancechange of the transmission path due to the presence of the polystyreneplate 43.

Also in the second embodiment, the gap of the THz-wave supplying unit isilluminated with femtosecond (fsec) laser light to generate THz wave,and the THz wave is transmitted through the transmission path. Afterinteraction with the sample in the porous material 42, the THz wavereaches the THz-wave detecting unit. Thus, the time-domain waveform andforth of the THz wave can be obtained.

In the second embodiment, the porous material 42 is in direct contactwith the line 41, and no intervening substance, such as adhesive, existstherebetween. Reliable detection can be expected in this embodiment.Further, the polystyrene plates 43 a and 43 b extend along the two sidesof the porous material 42 as described above. Accordingly, even whenmuch liquid sample beyond the liquid holding capability of the porousmaterial 42 is dripped, virtually no liquid sample spreads toward adirection leaving from the line 41. When the porous material 42 is ananisotropic porous material or fibrous material with properties thatliquid therein is hard to spread in a direction parallel with the line41, the liquid sample remains in the porous material 42. Accordingly, arelatively large liquid sample can be dripped at a time. Thus, thedistribution of the liquid sample can be more effectively regulated.

A third embodiment of the present invention will be described referringto FIGS. 5A to 5C. In this embodiment, the porous material 52 is setcrossing the line 51 by bonding or the like. For example, adhesive, suchas BCB and photoresist, is applied on a peripheral portion of the porousmaterial 52 near its two sides parallel with the line 51, and the porousmaterial 52 is bonded on the transmission path crossing the line 51. Thealignment mark can be provided for alignment at the time of bonding. Inthe third embodiment, the number of elements can be reduced. Otherstructures of the third embodiment are substantially the same as thoseof the first embodiment. Also in FIGS. 5A to 5C, reference numerals areomitted with respect to those elements.

Also in the third embodiment, the gap of the THz-wave supplying unit isilluminated with femtosecond (fsec) laser light to generate THz wave,and the THz wave is transmitted through the transmission path. Afterinteraction with the sample in the porous material 52, the THz wavereaches the THz-wave detecting unit. Thus, the time-domain waveform ofthe THz wave can be obtained, for example.

A fourth embodiment of the present invention will be described referringto FIGS. 6A to 6C. In this embodiment, a hole is formed in thepolystyrene plate 63 having a thickness of about 1 mm, and the porousmaterial 62 with an outer size slightly larger than the hole is setcovering the hole therewith, using bonding adhesive or the like. Thepolystyrene plate 63 is the non-infiltrative member of resin having ahigh transmissivity for THz wave. The size of the hole is about 0.4mm*0.3 mm, and the size of the porous material 62 is 0.6 mm*0.4 mm, forexample. Element 61 represents a metal line (signal line). Otherstructures of the fourth embodiment are substantially the same as thoseof the first embodiment. Also in FIGS. 6A to 6C, reference numerals areomitted with respect to those elements.

A flow path 64 can be formed in the polystyrene plate 63 surrounding theporous material 62, as illustrated in FIGS. 6A to 6C. The flow path 64extends to the side of the polystyrene plate 63. The size of the crosssection of the flow path 64 is about 0.05 mm*0.5 mm. In this case, theliquid sample is guided through an inlet port of the flow path 64 in theside of the polystyrene plate 63. The liquid sample dripped in the inletport of the flow path 64 by the micro-injector or the like reaches theporous material 62 due to capillary force and so forth of the flow path64. The liquid sample is infiltrated and held in the porous material 62due to its capillary force and so forth.

The flow path 64 can be connected to a reservoir or the like for storingthe liquid sample. In such a structure, the capillary of themicro-injector need not be accurately aligned with the porous material62 at the time of dripping the liquid sample, so the sample can bereadily introduced the porous material 62. Further, the possibility ofdamaging the porous material or the transmission path with the capillaryof the injector can be reduced.

Also in the fourth embodiment, similar to the first embodiment, the THzwave is transmitted through the transmission path. After interactionwith the sample in the porous material 62, the THz wave reaches theTHz-wave detecting unit. Thus, the time-domain waveform of the THz wavecan be obtained, for example.

A fifth embodiment of the present invention will be described referringto FIGS. 7A to 7C. In this embodiment, a second resin layer 73 is formedon a BCB layer 71 in the detecting apparatus including the THz-wavesupplying unit, the transmission path (microstrip line structure), andthe detecting unit. A hole 73 is formed in a portion of the second resinlayer 73 by plasma etching or the like. The second resin layer(non-infiltrative member) 73 is formed of a material (e.g., BCB) thatcannot infiltrate and hold the liquid sample therein. The second resinlayer 73 can be formed of photoresist, and the hole 74 can be formed byphotolithography or the like.

The size of the hole 74 is about the same as or slightly larger than thesize of the porous material 75. The porous material 75 is set in thehole 74. A gap can be formed between the porous material 75 and the edgeof the hole 74. In another configuration, the porous material 75 can bein contact with the edge of the hole 74. The thickness of the secondresin layer 73 can be larger or smaller than the thickness of the porousmaterial 75. The porous material 75 is set in the hole 74 with two sidesof the porous material 75 parallel with the line 72 being bonded byadhesive or the like. Other structures of the fifth embodiment aresubstantially the same as those of the first embodiment. Also in FIG. 7Ato 7C, reference numerals are omitted with respect to those elements.

In the fifth embodiment, the non-infiltrative member and the flow pathas described in the third and fourth embodiments can be fabricatedintegrally. Therefore, the number of the elements can be reduced in thefifth embodiment.

A sixth embodiment will be described with reference to FIGS. 8A to 8C.In the detecting apparatus of the sixth embodiment, the ground of thetransmission path is a metal plane 82 formed on a substrate 8 l byvacuum evaporation or the like. The substrate 81 is, for example, asilicon substrate. The metal plane 82 is made of, for example, 500 Åthin titanium and 3000 Å thin gold. On the metal plane 82, two LT-GaAslayers 83 a and 83 b are formed by epitaxial lift-off or the like. Thedielectric layer 86 transparent for THz wave is formed on the metalplane 82. Windows are then formed in portions on the two LT-GaAs layers83 a and 83 b, and a portion therebetween. The porous material 84 is setin the window formed on the two LT-GaAs layers 83 a and 83 b by thermalpressure bonding, adhesive bonding, or the like. The window in the resinlayer 86 is slightly larger than the porous material 84 so that theliquid sample can be supplied through the side of the porous material84. The porous material 84 is beforehand processed such that its surfaceopposite to the side of the substrate can be a smooth flat face withporosities thereon being packed. Alternatively, a resin film or the likeis attached on the above surface of the porous material 84.

The metal line 85 is formed on the surface of the above structure byvacuum evaporation or the like. The metal plane 82, the porous material84 and the line 85 constitute the transmission path of the microstripline type. In this embodiment, the porous material 84 serves as thedielectric layer of the transmission path, too. Other structures of thesixth embodiment are substantially the same as those of the firstembodiment. Also in FIGS. 5A to 8C, reference numerals are omitted withrespect to those elements.

In the above structure, a glass capillary 86 is placed near the side ofthe porous material 84 to supply the liquid sample thereby. The liquidsample is thus infiltrated in the porous material 84. The flow path canbe used in place of the glass capillary.

In this embodiment, the porous material 84 is provided in the dielectriclayer of the transmission path, so that the liquid sample can besupplied to a predetermined location in a strong electric fielddistribution with a predetermined volume. Also in the sixth embodiment,the THz wave is transmitted through the transmission path. Aftereffective interaction with the sample in the porous material 84, the THzwave reaches the THz-wave detecting unit. Thus, the time-domain waveformof the THz wave can be accurately obtained, for example.

A seventh embodiment will be described. In the detecting apparatus ofthe seventh embodiment, the liquid sample infiltrated in the porousmaterial is cooled and frozen. When the liquid sample containingvolatile liquid is infiltrated in the porous material, the liquidevaporates with time, and only non-volatile constituent remains in theporous material. The result of detection of the liquid sample varieswith time since the evaporation proceeds with time. Further, time to berequired for the evaporation and moisture content of the sample afterthe evaporation vary depending on ambient conditions, such astemperature and humidity of a place where the liquid sample exists.

To overcome such problem and acquire a stable result of detection, theliquid sample infiltrated in the porous material is cooled and frozen.Freezing is performed at such a timing that the condition of the sampleto be detected cannot vary with time. The freezing can be conducted by amethod of blowing cold (e.g., coolant and liquid nitrogen) on themeasurement sample to directly freeze the sample, or a method of coolingthe transmission path to indirectly freeze the sample using a Peltierdevice or the like, for example.

At the time of freezing, the porous material, such as the membranefilter, also serves as a heat insulating material for preventing heatfrom flowing into the frozen sample from outside.

This embodiment can be employed for detection of a material contained inliquid from a living object. For example, sweat or blood is infiltratedand frozen in the porous material, and alcohol, sugar or the liketherein is measured. Further, it is possible to infiltrate and freeze,in the porous material, a volatile liquid containing fine particleswhose diameter are below the porosity diameter of the porous material,and detect such particles.

As described in the foregoing, according to the present invention, it ispossible to provide devices using electromagnetic waves at frequency orfrequencies in a range between 30 GHz and 30 THz.

Except as otherwise disclosed herein, the various components shown inoutline or in block form in the figures are individually well-known andtheir internal construction and operation are not critical either to themaking or using of the present invention or to a description of the bestmode of the invention.

While the present invention has been described with respect to what ispresently considered to be the embodiments and examples, it is to beunderstood that the invention is not limited to the disclosedembodiments and examples. The present invention is intended to covervarious modifications and equivalent arrangements included within thespirit and the scope of the appended claims.

This application claims priority from Japanese Patent Application No.2006-108563, filed Apr. 11, 2006, and Japanese Patent Application No.2007-023610, filed Feb. 2, 2007, the contents of which are herebyincorporated by reference.

1.-11. (canceled)
 12. A detecting apparatus for detecting information ofa liquid object, the detecting apparatus comprising: a transmissionpath; an electromagnetic-wave supplying unit for supplying anelectromagnetic wave in a frequency range between 30 GHz and 30 THz tothe transmission path; an electromagnetic-wave detecting unit fordetecting the electromagnetic wave transmitted through the transmissionpath; and an infiltrative holding member for infiltration and holding ofthe liquid object, wherein the infiltrative holding member is set at alocation containing at least a portion in which an electric fielddistribution of the electromagnetic wave propagating along thetransmission path extends, wherein a non-infiltrative member which isincapable of infiltrating the liquid object is set partly or entirelysurrounding the infiltrative holding member, wherein a spacing, whosesize is less than a wavelength of the electromagnetic wave supplied bythe electromagnetic-wave supplying unit, is present between theinfiltrative holding member and the non-infiltrative member, and whereina structure of the infiltrative holding member is anisotropic and acharacteristic direction of the infiltrative holding member is alignedwith an extending direction of the transmission path
 13. The detectingapparatus according to claim 12, wherein the transmission path comprisesa metal.
 14. The detecting apparatus according to claim 12, wherein theinfiltrative holding member comprises one of a fibrous material and aneedle structure.
 15. A detecting apparatus for detecting information ofa liquid object, the detecting apparatus comprising: a microstriptransmission line made of metal; an electromagnetic-wave supplying unitfor supplying an electromagnetic wave in a frequency range between 30GHz and 30 THz to the transmission line; an electromagnetic-wavedetecting unit for detecting the electromagnetic wave transmittedthrough the transmission line; an infiltrative holding member forinfiltration and holding of the liquid object; and a substrate having awindow, wherein the infiltrative holding member is provided in thewindow, wherein the infiltrative holding member is set at a locationcontaining at least a portion in which an electric field distribution ofthe electromagnetic wave propagating along the transmission lineextends, and wherein the metallic microstrip transmission line isprovided on the infiltrative holding member.
 16. A detecting apparatusaccording to claim 15, wherein a size of the window is larger than asize of the infiltrative holding member and the liquid object can besupplied from a side surface of the infiltrative holding member.
 17. Adetecting apparatus for detecting information of a liquid object, thedetecting apparatus comprising: a substrate; a transmission line; anelectromagnetic-wave supplying unit for supplying an electromagneticwave in a frequency range between 30 GHz and 30 THz to the transmissionline; an electromagnetic-wave detecting unit for detecting theelectromagnetic wave transmitted through the transmission line; aninfiltrative holding member provided on the transmission line forinfiltration and holding of the liquid object; and a non-infiltrativemember which is incapable of infiltrating the liquid object, wherein theinfiltrative holding member is set at a location containing at least aportion in which an electric field distribution of the electromagneticwave propagating along the transmission line extends, wherein thenon-infiltrative member is set partly or entirely surrounding theinfiltrative holding member, wherein the transmission line is amicrostrip line made of a metal, and wherein an optical constant of thenon-infiltrative member for the THz wave transmitted through thetransmission line is approximately equal to that of the infiltrativeholding member.
 18. A device comprising: a substrate; a transmissionpath provided on the substrate for transmitting an electromagnetic wavein a frequency range between 30 GHz and 30 THz; an electromagnetic wavesupplying unit for supplying the electromagnetic wave to thetransmission path; an electromagnetic wave detecting unit for detectingthe electromagnetic wave transmitted through the transmission path; andan infiltrative holding member provided on the transmission path forinfiltrating and holding a liquid object, wherein the infiltrativeholding member is disposed such that the electromagnetic wavetransmitted through the transmission path and the liquid object interactwith each other, and wherein a structure of the infiltrative holdingmember is substantially isotropic.
 19. A device according to claim 18,wherein a shape of the infiltrative holding member is asymmetrical withrespect to the transmission line and the infiltrative holding member isdisposed such that an electric field distribution of the THz wavetransmitted through the transmission line is substantially symmetricalwith respect to the transmission line.
 20. A device according to claim18, wherein the infiltrative holding member comprises one ofpolypropylene, polysulfone, nylon and polyethersulfone.
 21. Thedetecting apparatus according to claim 12, wherein the non-infiltrativemember is set partly or entirely surrounding the infiltrative holdingmember at a position perpendicular to the electromagnetic wavetransmitted through the transmission path and at a position where theliquid object is infiltrated.
 22. The device according to claim 18,further comprising non-infiltrative members which are incapable ofinfiltrating the liquid object, wherein the transmission path isdisposed between the non-infiltrative members provided on the substrate.